Team:Aix-Marseille/Notebook WIP

Monday, June 13th

• Preparation of heat competent DH5α cells

Tuesday, June 14th

• Transformation of DH5α cells with test plasmid

Wednesday, June 15th

• Preparation of heat competent Tg1 cells
• Transformation of Tg1 cells with test plasmid

Thursday, June 16th

• Transformation of DH5α cells with pSB1C3 + RFP/li>
• Transformation with K880005, K823017, I0500, K1404006, B0034

Friday, June 17th

• Miniprep K880005, K823017, I0500, K1404006, B0034
• Miniprep pSB1C3 + RFP

Monday, June 20th

• pSB1C3 digestion from 17/06 miniprep
• pSB1C3 digestion gel migration : NO PHOTO
• Biobricks minipreps gel migration : OK PHOTO
• Transformation Tg1 with RFP+pSB1C3

Tuesday, June 21th

• pSB1C3 digestion and gel migration, NucleoSpin gel purification and PCR cleanup
• FliC E.Coli and FliC Desulfo digestion E/P and ligation with pSB1C3
• E. Coli and Desulfo FliC ligation in linearized pSB1C3
• Transformation of pSB1C3 RFP in Tg1

Wednesday, June 22th

• Transformation in DH5α of :
  • FliC coli/desulfo in pSB1C3
  • Linearized pSB1K3
  • Linearized pSB1A3
  • Linearized pSB1T3
  • Linearized pSB1C3
• Miniprep B0034
• Transformation in DH5α of interlab studies plasmids

Thursday, June 23th

• Digestion E/P pSB1C3
• Purification pSB1C3 linéarisé purifié 23/06
• Digestion E/P of DesA, DesB, DesC, DesD
• 3A Assembly of B0034 and I0500 in pSB1K3
• Digestion with pSB1C3 Des 23/06

Friday, June 24th

• Digestion E/P pSB1C3, pSB1A3, pSB1K3, pSB1T3, and agarose gel purification
• Colony PCR of
  • RBS+promoter
  • GFP
  • empty
• Ligation: FliC E.Coli, FliC desulfo, DesA B C D
• Ligation pSB1C3 and fliC E.Coli / desulfo digested E/P 21/06
• Transformation in Tg1 and DH5α
• Redone transformation test device 1 and 3 interlab studies
• Miniprep pSB1C3 A3 K3 T3