Team:ASIJ Tokyo/Parts

Parts

Anderson Promoters

Strength: weak (0.33) BBa_J23110

• Wanted a weak promoter to measure its PET plastic degradation up against the other promoters

Strength: moderate (0.58) BBa_J23111

• Wanted a moderate promoter to measure its PET plastic degradation up against the other promoters

Strength: strong (1) BBa_J23100

• Wanted a strong promoter to measure its PET plastic degradation up against the other promoters

N-OsmY tag: Fusion proteins with an N-terminal osmY have been shown to successfully secrete proteins of interest

C-Myc tag: Make our PETase construct easier to detect for Western Blot to assay expression and secretion

PETase sequence: Needed the enzyme to degrade PET plastic

Plasmid backbone: pSB1C3: Best vector to move our ligated DNA produced in Gibson Assembly

High efficiency e. Coli cells: Were the host for our optimized PETase