Team:UofC Calgary/Composite Part

iGEM Calgary 2016

Composite Parts

The backbone of the U of Calgary project are the composite parts. The composite parts are an amalgamation of different registry parts which act together to provide a novel function. The composite parts designed for this project are based around the Bowman-Birk Protease inhibitor (BBI) and the ComK part that was submitted to the registry by the 2014 Calgary team.

A variety of different parts were used across all of the BBI composite constructs. We used Pveg, a constitutive promoter, and double terminators to book end the rest of the internal genes. To improve secretion of BBI from B. subtilis, we added a secretion tag as well as a transdermal (TD1) tag. The secretion tag is a localization tag for the Sec pathway in B. subtilis

Composite Parts

BBa_K2008001

E. coli – GFP-BBI 5
BBI with N-terminal KSCI solubility tag fused to GFP
Registry Link

BBa_K2008002

B. subtilis– pVeg -> sec-> TD1-> BBI-GFP (BBI 1 GFP w/o)
Registry Link

BBa_K2008003

B. subtilis – pVeg->sec-TD1-KSCI-BBI-GFP (BBI 5 GFP w/o)
Registry Link

BBa_K2008004

B. subtilis – secretion tag, transdermal tag, and BBI without internal restriction sites (BBI 1 w/o)
Registry Link

BBa_K2008005

B. subtilis – secretion tag, transdermal tag, solubility tag and BBI without internal restriction sites (BBI 5 w/o)
Registry Link

BBa_K2008006

B. subtilis - Improved ComK: removal of internal restriction sites
Registry Link

BBa_K2008007

B. subtilis - Improved ComK: addition of AmyE homology
Registry Link

BBa_K2008008

B. subtilis - Bacillus subtilis modular secretion platform with ThrC homology for chromosomal integration
Registry Link

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