Team:IIT-Madras/Composite Part

Composite Parts

We made and submitted a total of 21 parts in standard plasmid backbone, pSB1C3. Our composite parts can be summarized into following categories:

1. Promoter-RBS parts: We have made a combination of promoter-RBS parts, R0011 with B0030/31/32/34. Part ranges from BBa_K1856001-BBa_1956004.
2. Dual fluorescence reporter devices: We have made six devices ranging with different promoter-RBS parts producing Gfp and Rfp proteins. Part ranges from BBa_K1956014-15, BBa_K1956017-18, BBa_K1956022-23.

Igem dual reporter.png

3. RIBOS devices: We have cloned RIBOSON, [http://parts.igem.org/Part:BBa_K1956019 BBa_K1956019] and RIBOSOFF, [http://parts.igem.org/Part:BBa_K1956005 BBa_K1956005] [http://parts.igem.org/Part:BBa_K1956020 BBa_K1956020], [http://parts.igem.org/Part:BBa_K1956021 BBa_K1956021] devices to test their strength and functionality.

Iitm ribos construct.png

Best Composite Part

Dual expression vector, [http://parts.igem.org/Part:BBa_K1956016 BBa_K1956016], device has been made to enable user clone their promoter & RBS parts with this device to achieve efficient characterization, i.e. noise and strength, for their parts and devices.

Express vector.png