Team:IIT-Madras/Composite Part
Composite Parts
We made and submitted a total of 21 parts in standard plasmid backbone, pSB1C3. Our composite parts can be summarized into following categories:
1. Promoter-RBS parts: We have made a combination of promoter-RBS parts, R0011 with B0030/31/32/34. Part ranges from [http://parts.igem.org/Part:BBa_K1956001 K1956001]-[http://parts.igem.org/Part:BBa_K1956004 K1956004].
2. Dual fluorescence reporter devices: We have made six devices ranging with different promoter-RBS parts producing Gfp and Rfp proteins. Part ranges from [http://parts.igem.org/Part:BBa_K1956014 BBa_K1956014]-[http://parts.igem.org/Part:BBa_K1956015 15], [http://parts.igem.org/Part:BBa_K1956017 BBa_K1956017]-[http://parts.igem.org/Part:BBa_K1956018 18], [http://parts.igem.org/Part:BBa_K1956022 BBa_K1956022]-[http://parts.igem.org/Part:BBa_K1956023 23].
3. RIBOS devices: We have cloned RIBOSON, [http://parts.igem.org/Part:BBa_K1956019 BBa_K1956019] and RIBOSOFF, [http://parts.igem.org/Part:BBa_K1956005 BBa_K1956005], [http://parts.igem.org/Part:BBa_K1956020 BBa_K1956020], [http://parts.igem.org/Part:BBa_K1956021 BBa_K1956021] devices to test their strength and functionality.
Best Composite Part
Dual expression vector, [http://parts.igem.org/Part:BBa_K1956016 BBa_K1956016], device has been made to enable user clone their promoter & RBS parts with this device to achieve efficient characterization, i.e. noise and strength, for their parts and devices.
