Team:Technion Israel/Measurement
Introduction
Although there is an abundant number of chemotaxis assays available today, most of them were designed
50 to 60 years ago and almost none provide a real time measurement without the use of fluorescence
labeling, for example FRET test.
The use of Porous Si (PSi) and oxidized PSi (PSiO2) matrices for biological sensing is on the rise.
So far various analytes such as DNA, proteins and bacteria have been proven to be detectable on such
matrices. The common method to monitor the interaction of said analytes within the porous films is
reflective interferometric Fourier transform spectroscopy (RIFTS), as it allows a real time measurement
and output for the user.
Here we present the results of an early experiment for the detection of chemotactic activity on the
porous silicon films initially developed for bacterial detection.
Fig. 1: ???.
References:
1. Reyes-Darias, J.A., Yang, Y., Sourjik, V., and Krell, T. (2015). Correlation between signal input and output in PctA and PctB amino acid chemoreceptor of Pseudomonas aeruginosa. Mol. Microbiol. 96, 513–525.
