Team:CU-Boulder/Composite Part

• Home
• Project
  • Description
  • Background
  • Experiments
  • Results
  • Notebook
• Team
  • Team
  • Collaborations
  • Attributions
• Parts
  • Parts
  • Basic Parts
  • Composite Parts
  • Part Collection
• Safety
• Human Practices

Composite Part

G26-EutS Mid (BBa_K2129002) is a mutated version of EutS (BBa_K2129001) with an Amber stop codon in place of Glycine 26. This is designed to enable an amber-codon recognition tRNA to insert non-canonical amino acids at this locus during translation of the EutS protein. This residue location, for the non-canonical amino acid phenylalanine-4’-azobenzene, seems to result in variable cage formation, which may be indicative that this protein can be used as a light-activated open/closed nanocompartment. This part could see a wide variety of uses.

Mutant G26-Amber EutS with no available AzoPhe

Mutant G26-Amber EutS with .1% AzoPhe

1C3 Plasmid map for G26-Amber EutS