Team:CU-Boulder/Part Collection

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Part Collection

EutS mid and low (BBa_K2129004, BBa_K2129005) express EutS proteins at a medium and low level respectively, which has been characterized to be the best levels of expression for high levels of visible microcompartments. eGFP low (BBa_K2129006) is the best fluorescent protein expression level for visualizing these compartments in the cell, but future teams could use eGFP Pbad (BBa_K2129007) to achieve variable expression levels as seen fit. This collection is an excellent demonstration of the properties of EutS and its ability to localize proteins tagged with EutC 1-19.

eGFP Low Expression with Euts Mid Expression

EutS Mid Plasmid Map (1C3)

eGFP Low Expression with EutS Low Expression

EutS Low Plasmid Map (1C3)

eGFP Pbad Expression with EutS Low Expression (Arabinose 5%)

EutC eGFP Low Plasmid Map (1C3)

eGFP Pbad Expression with EutS Low Expression (Arabinose 0%)

EutC eGFP Pbad Plasmid Map (1C3)