Team:Toronto/Notebook-w06-mon
console.js:26
Monday June 20, 2016
Monday, 6/20
Members Present: Alex, Cathy, Kat, Hamed, Marc, Celene, Dk, Andrea
LAB:
Morning:
- Ran gel electrophoresis protocol using RFP and various amounts of DNA ladder found in the fridge (3ul, 6ul, 10ul) [picture to follow]
●
Concluded that 6uL of DNA ladder should be sufficient.
Afternoon:
Meeting with Dr. Pardee & Christian Euler
Administrative:
TO DO:
For the next day:
LAB TEAM:
LAB MANAGERS:
- Please buy a USB! (something cheap will do)
- Check the prices for the S30/S12 reagents (and if we have them in our inventory or the Biozone inventory):
●
ATP; GTP, UTP, CTP;
●
L-5-formyl-5, 6, 7, 8-tetrahydrofolic acid (folinic acid);
●
potassium glutamate;
●
ammonium glutamate;
●
magnesium glutamate;
●
20 amino acids (is there a mix?);
●
L-[14C(U)]-leucine;
●
nicotinamide adenine dinucleotide (NAD);
●
coenzyme-A (CoA);
●
spermidine;
●
putrescine;
●
sodium oxalate;
●
phosphoenolpyruvate (PEP);
●
DTT
●
NaH2PO4
●
Na2HPO4
A | B | C | D | E | |
1 | MONDAY | TUESDAY | WEDNESDAY | THURSDAY | FRIDAY |
2 | Gel electrophoresis on RFP | Rubidium chloride competent cells | |||
3 | Restriction enzyme digest (using the iGEM protocol) | ||||
4 | Gel electrophoresis | ||||
Table1
Gmail correspondence:
Meetings/Notes:
Notes from General Meeting:
References:
