Team:Toronto/Collaborations
Collaborations
iGEM Concordia
We collaborated with iGEM Concordia to validate the functionality of our constructs BBa_K2048001 and BBa_K2048002. We have shipped our DNA samples to Concordia iGem on September 2016. Following protocol was executed by iGem Concordia for the preliminary results:
- Transformed E.Coli DH5a cells with BBa_K2048001 and BBa_K2048002 separately.
- For control groups untransformed E.Coli DH5a cells were used.
- 10uM and 100uM gold(III) metal ions were added to the control and experimental groups along with XGal
- Blue color change was observed.
Results:
After the 2-3 hours, visible blue color was observed in 10uM and 100uM tubes containing E.Coli BBa_K2048001. Faint blue color was also observed in 10uM and 100uM tubes containing E.Coli BBa_K2048002.
After 24h, tubes with BBa_K2048002 device also showed dark blue color. In addition, BBa_K2048002 tubes had a darker visible blue compared to BBa_K2048001.
Concordia iGEM teams results further validated the functionality of our devices BBa_K2048001 and BBa_K2048002. Moreover, they proved the expression levels can be observed at 100uM gold(III) levels in presence of XGal.
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