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Wednesday June 15, 2016

Wednesday, 6/15

fMembers Present: Kat, Hamed, Dk, Tam, Alex, Cathy, Andrea

LAB:

Morning:

- Preparing a new set of chemically competent cells (B)

- E-mailed Kayla asking for lysis protocol

- Transformation/competent cell protocol hasn't worked with competent cells batch A:

0.5pg triplicates streak plated & one plated with sterile beads. No growth

5pg triplicates streak plated & one plated with sterile beads. 4 colonies on one of the triplicates.

10pg triplicates steak plated & one plated with sterile beads. 8 colonies on one of the triplicates.

20pg triplicates streak plated & one plated with sterile beds. 2 colonies on one of the triplicates and 2 colonies for the plate with beads on it.

50pg triplicates streak plated & one plated with sterile beads. 2 colonies on one of the triplicates and 4 colonies for the plate with beads on it.

Afternoon:

- Performing transformation efficiency protocol on the old and the new batch of chemically competent cells

Administrative:

- Officialized HPLC draft proposal

- Summarized GolS protein function

TO DO:

- Make LB+CAM (+agar) plates

- Miniprep

For the next day:

LAB TEAM: Perform cell lysis using sonication (follow up with Kayla)

LAB MANAGERS: Figure out liquid nitrogen safety and transfer for tomorrow (transfer bucket?)

Gmail correspondence:

- Contact Dr. Thomas O'Halloran about his entire protocol on MerR family purification: t-ohalloran@northwestern.edu

Meetings/Notes:

Team:Toronto/Notebook-w05-wed