Team:Toronto/Notebook-w03-tue

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Week 3: May 31 2016

Tuesday, 5/31
Members present: Katariina, Karim, Celine, Bohdan, Hamed
Lab Stuff: (research, plasmid design, protocol design, troubleshooting)
UNS plasmid construct:
Plasmid Design:
UNS overview plasmid constuct.png
thumbnail
Sample plasmid showing the positioning of UNS for our construct. Protein X and protein Y are just random examples.
A
B
1
Merging constructsLigating Primer
2
Protein X - Protein YUNS6 - UNS5
3
Protein Y - BackboneUNS1-UNS4
4
Backbone - Protein XUNS9 - UNS8
5
Protein Y - BackboneUNS5-UNS 9
6
Backbone - Protein XUNS4 - UNS6
Table1
Proposal update:
Created an updated protocol for one of our investors, excluding FBA from the computational team, and removing Biomineralization aspects of Wet Lab team.
Proposal for Rob M .pdf
Protocol Design:
Inputted protocol for Spephadex G-25 Column chromatography,
Administrative: (shipment orders, inventory)
Email Updates: (Correspondance with proffessors, shipping companies, etc)
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Sent a revised proposal to Rob M.