Team:Toronto/Experiment-TB (Terrific Broth) Buffer

TB (Terrific Broth) Buffer · Benchling

TB (Terrific Broth) Buffer

Introduction

Terrific broth (TB, not to be confused with T Broth)

Materials

    • 12 g Bacto tryptone
    • 24 g Bacto yeast extract
    • 4 mL Glycerol
    • 12.54 g of K2HPO4 (potassium phosphate dibasic)
    • 2.32 g of KH2PO4 (potassium phosphate monobasic)
    • 100 mL Milli-Q H2O
    • 900 mL distilled H2O

Procedure

  • Potassium phosphate solution:
  1. Prepare 100 mL of 12.54 g of K2HPO4 , 2.32 g of KH2PO4 and mili-Q H2O in a graduated cylinder up to 100 mL.
  • TB
  1. Mix tryptone, yeast extract and glycerol, and add distilled water up to 900mL.
  1. Pour into 2 L flask (or greater)
  1. Autoclave (liquid cycle) both the potassium phosphate solution and TB buffer.
  1. Allow liquid to cool to less than 60 degrees Centrigrade.
  1. Add both the potassium phosphate solution and TB buffer together.



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