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Our first day in the lab, where we found our beloved starter kit. The following 2 weeks were focused on literature research, ordering of reagents and kits, lab preparation and finding sponsors for our project. The team also received training on Benchling and MoClo, which we expect we are going to use very frequently in the next few months. The rhodococcus team started growing Rhodoccocus jostii on plates. The synechocystis team received the pPMQAK1 plasmid to be used for cloning in E. coli before transformation into Synechocystis. Team MoClo: Received CIDAR MoClo plasmids from Laura Tuck Penicillium team?
After much deliberation, we finally decided to use the Bba_ P10500 Phytobrick Universal Acceptor Vector as our destination vector for all our (level 0) MoClo compatible DNA parts. The order for the synthesis of DNA gblocks for our parts was placed through IDT. Team MoClo: Finished Primer design for mutagenesis & start cloning plasmids for template
Our first MoClo assembly was finally performed using DNA parts found in the CIDAR MoClo kit. However, very few colonies were obtained and therefore our MoClo reaction mixture and conditions were adapted. Team MoClo: Mutagenesis & screening of illegal removal sites for Rhodococcus vectors. Mut2 and Mut3 showed a good result.
We are the University of Edinburgh Overgraduate iGEM Team, competing in the new application track in iGEM 2016. read more
School of Biological Sciences The University of Edinburgh King's Buildings Edinburgh EH9 3JF, United Kingdom
Email: edigemmsc@ed.ac.uk
