Team:IIT-Madras/Proof
Noise in Devices
RIBOS
RIBOSON
We designed our experiment in following way: Trigger RNA: A part of RNA molecules, which codes for [http://parts.igem.org/Part:BBa_E1010 RFP] protein. Switch RNA: Switch RNA was designed to repress the translation process by masking the RBS placed upstream of GFP protein coding part. After interacting with the RNA molecules of RFP gene, RIBOSON would allow the RBS to open up and bind to ribosomal machinery and protein synthesis takes place.
RIBOSOFF
Trigger RNA: A part of RNA molecule, which codes for lacI protein. Switch RNA: Switch RNA was designed to activate the translation process by allowing the ribosomal machinery to bind to RBS, placed upstream of GFP protein coding part. After interacting with the RNA molecules of lacI gene, RIBOSOFF changes it's confirmation to mask the RBS and represses the translation process.
