Team:Newcastle/Notebook/Diary

4th July

09:00-17:00:

  • COMSOL Training Day

iGEM Week 3

5th July

09:00-13:00:

  • Interlab Study

14:00-16:00:

  • COMSOL and Action Points

16:00-17:00:

  • Seminar on “Synthetic Biology: perspectives from artificial life”

iGEM Week 3

6th July

09:00-09:30:

  • Checked the plate reader

09:30-12:00:

  • Action Points

12:00-13:00:

  • Lunch

13:00-14:00:

  • Skyped Chennai

14:00-15:00:

  • Planned for the meeting

15:00-17:00:

  • Meeting

iGEM Week 3

7th July

09:30-11:00:

  • Start prep for the plasmids to be sent to DNA2.0

11:00-12:00:

  • Action Points

12:00-13:00:

  • Lunch

13:00-14:30:

  • Continued prepping cultures for freezing and for the plasmids to be sent to DNA2.0

14:30-15:30:

  • Ollie and Lauren filmed for the School of Biology

15:30-17:00:

  • Action Points

iGEM Week 3

8th July

09:00-12:00:

  • Mini prepped Plasmids to be sent to DNA2.0

12:00-13:00:

  • Lunch

13:00-14:00:

  • Measured spectrophotometry the plasmids and packaged up for DNA2.0

14:00-16:00:

  • Worked on the Action Points

16:00-17:00:

  • Meeting with the Patrick Degenaar

27th June

09:00-13:00:

  • FabLab Sunderland

14:00-15:00:

  • Lunch

14:00-15:00:

  • Met with Andrew Filby in regards to flowcytometry

15:00-16:00:

  • Sourced components for the fuel cell

14:00-17:00:

  • Planned and completed action points

iGEM Week 2

28th June

09:00-12:00:

  • Made Microbial Fuel Cell

10:00-12:00:

  • Liquid cultures were made and then left in the fridge overnight

12:00-13:00:

  • Lunch

13:00-17:00:

  • Breadboard designed /Light bulb construct redesigned/ Wiki design looked at etc.

iGEM Week 2

29th June

09:00-13:00:

  • Planned/ Checked Fuel Cell

13:00-15:00:

  • COMSOL licences tested

14:00-15:00:

  • Incubated liquid cultures

15:00-17:00:

  • Meeting

18:00-19:00:

  • MAKERSPACE

iGEM Week 2

30th June

09:00-11:00:

  • Interlab study was discussed with Tom

11:00-12:00:

  • Lightbulb design construct was run through with Jem

12:00-14:00 :

  • Lunch / Exchanging the Euro

14:00 - 15:00:

  • More cultures grown up for the interlab/ IWBDA booked and Paris tickets picked up and confirmed.

15:00 - 17:00:

  • Protocols started

iGEM Week 2

1st July

12:00-21:00:

Travelling to Paris

iGEM Week 2

2nd July

09:00 - 12:00:

  • Exhibition Stands in Paris

12:00-14:00:

  • Lunch Break

14:00-15:30

  • Roundtable 1 - Synthetic Biology: Challenges and Risks

    • François Képès - Research Director at CNRS, founding director of the Epigenomics Project an iSSB

    • Héloïse Muller - PhD at Institute Pasteur

    • Andrew Tolonen - Leader of Tolonen group at the Genoscope-CEA

    • Christophe Genisset - Security Officer at SGDSN (French Secretariat for Defence and National Security)

    • Alexei Grinbaum - Researcher at LARSIM, the Philosophy of Science Group at CEA-Saclay

15:30-16:00:

  • Coffee Break

16:00-17:30:

  • Roundtable 2 - Synthetic Biology: A New Economic World

    • Philippe Jais - Chief Executive and Scientific Officer at Eukarys

    • Cyrille Pauthenier -President of Abolis Biotechnologies

    • Samuel Juillot - CSO at Glowee (In absentia - sent a PhD student in his place)

    • Michael Krel - CEO at Enobraq

17:30-18:00:

  • Talk from Randy Rettberg about iGEM and its importance in the world

21:00-03:00:

  • Party!!!

iGEM Week 2

3rd July

09:00 - 18:00:

  • Site-seeing around Paris

18:00 - 02:10:

  • Travelling Home

20th June

Half the group

Kristina, Ollie and Josh

  • Made streak plates of all the colonies for the interlab – left to incubate over the weekend

  • Made liquid cultures

 

Other half the group

Jake, Emilija, Ollie, Kristina, Kerry, Rupert and Lauren

  • Looked at papers

  • Planned the week

  • Wrote the agenda minutes

  • Started a plan for Wiki

Training

Josh

  • Josh was introduced to lab techniques that the rest of the team at worked on the previous week, such as using the sterile hood, fire drills and safety techniques.

iGEM Week 1

21st June

09:00-11:00:

  • Wiki Ideas/Themes and Layout

    • Idea for baseball themed cards for the team

    • Old coke adverts as a colour scheme and theme

11:00 - 12:00:

  • Lecture in using COMSOL and MATLAB by Dana Ofiteru

12:00-13:00:

  • Lunch

13:00-13:30:

  • Check the streak plates

13:30 - 16:00:

  • Split into sub-groups to research about:

    • Beyond the bench

    • Light Bulb component of the breadboard

16:00-17:00:

  • Skyped with Edinburgh

    • Possible collaboration

iGEM Week 1

22nd June

09:00-11:00:

Jake and Kristina

  • Analysed the Interlab Results

Ollie and Josh

  • Researched papers on fuel cells and components for the light bulb.

Emilija and Lauren

  • Researched components for the light bulb

  • Tried to construct some BioBricks on Benchling

Rupert

  • Designed the Nav Bar for the Wiki

Kerry

  • Worked on the project description for the Wiki

  • Deadline: 1st July

11:00 – 11:30:

Ollie and Josh

  • Plated up the Positive Control from the previous day for the team to repeat the interlab study

12:00-13:00:

  • Lunch

13:00-14:30:

  • Met with Professor Ian Head, Dr Ed Milner, Paniz Izadi from the School of Civil Engineering and Geosciences to discuss microbial fuel cells

14:00 - 15:00:

  • The Light sub-group met with supervisors to design the Killer BioBrick

15:00-17:00:

  • Meeting with supervisors (See Agenda and Minutes for the 22nd June)

iGEM Week 1

23rd June

09:00-11:00:

  • Finalised Killer BioBrick construct

11:00-12:00:

  • Modelling Lecture

12:00 - 12:30:

  • Lunch

12:30-14:00:

  • BioNetLab Training in modelling BioBricks

14:00-17:00:

  • Attempted to model our Killer BioBrick using RuleBender

iGEM Week 1

24th June

09:30-10:30:

  • Brexit Breakfast

10:30-11:00:

  • Check with Jem about Benchling design

11:00-12:00:

  • Looked at the Action Points, crossed off the ones we had done and assigned people to complete the other action points.

12:00-17:00:

  • Assigned tasks were carried out