Team:NYMU-Taipei/Notebook-Lab Book-pBAR pMCL1 EL222 TtrpC

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8/16

  • pBAR_EL222_TtrpC transformed into competent cell

  • pBAR_EL222_TtrpC containing cultures used 2 in 1

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    8/17

  • pBAR_EL222_TtrpC extracted and attempted to amplify with plasmid PCR------we did not amplify the target sequence (Hence, we tried ligation again)

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    8/18

  • pBAR_EL222_TtrpC transformed into competent cell

  • pBAR_EL222_TtrpC containing cultures used in 3 in 1

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    8/19

  • pBAR_EL222_TtrpC extracted from transformed cultures, checked with restriction enzyme digestion and plasmid PCR (failure)

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    8/28

  • pVP-EL222 and TtrpC PCR ligation ------- NC had bands

  • pVP-EL222 and TtrpC PCR ligation ------- used remixed primer

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    8/29

  • pVP-EL222 and TtrpC PCR ligation ------- the concentration was not high enough after gel extraction

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    8/31

  • pVP-EL222 and TtrpC PCR ligation

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    9/1

  • pVP-EL222 and TtrpC PCR ligase

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    9/2

  • EL222-TtrpC digestion

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    9/3

  • pBAR_EL222_TtrpC transformed into competent cell

  • pBAR_EL222_TtrpC containing cultures used in 3 in 1

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    9/5

  • pBAR_EL222_TtrpC checked with restriction enzyme digestion

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    9/6

  • EL222-TtrpC digestion

  • pBAR_EL222_TtrpC checked with restriction enzyme digestion

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    9/7

  • pBAR_EL222 PCR

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    9/16

  • pBAR_EL222_TtrpC did plasmid PCR

  • pBAR_EL222_TtrpC did plasmid PCR (confirmed successfully)

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    9/17

  • pBAR_EL222_TtrpC plasmid stored and transformed cultures cryopreserved

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