Team:NYMU-Taipei/Notebook-Lab Book-pBAR pMCl1 RFP TtrpC

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8/17

  • pBAR _RFP_TtrpC transformed into competent cell

  • pBAR _RFP_TtrpC containing cultures used in 2 in 1

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    8/18

  • pBAR _RFP_TtrpCdid extracted from transformed cultures and checked with restriction digestion

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    8/19

  • pBAR _RFP_TtrpC checked with restriction digestion------the incorrect bands appeared in gel electrophoresis results

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    8/27

  • RFP and TtrpC PCR ligation

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    8/28

  • RFP_TtrpC digested for ligation with pBARGPE1------ the concentration was not high enough

  • RFP and TtrpC did PCR to ligase(failure, we change the volume of the primer and Mg2+)

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    8/31

  • RFP_TtrpC digested for ligase with pBARGPE1 (the band existed on wrong site)

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    9/1

  • RFP_TtrpC digested

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    9/2

  • pBAR _RFP_TtrpC transformed into competent cell

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    9/4

  • pBAR _RFP_TtrpC did plasmid PCR

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    9/5

  • pBAR _RFP_TtrpC transformed into competent cell ------ there was no E.coli on the first plate

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    9/6

  • pBAR _RFP_TtrpC transformed into competent cell

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    9/7

  • pBAR _RFP_TtrpC transformed into competent cell

  • pBAR _RFP_TtrpC did 2 in 1

  • pBAR _RFP_TtrpC did 3 in 1

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    9/8

  • pBAR _RFP_TtrpC plasmid PCR

  • pBAR _RFP_TtrpC digestion check

  • pBAR _RFP_TtrpC digestion check

  • pBAR _RFP_TtrpC did plasmid extraction

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    9/9

  • pBAR _RFP_TtrpC did digestion check

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    9/10

  • RFP and TtrpC use primer to ligase

  • RFP_TtrpC PCR to amplify the target sequence

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    9/13

  • pBAR _RFP_TtrpC transformed into competent cell

  • RFP_TtrpC PCR to amplify the target sequence

  • pBAR _RFP_TtrpC did 3 in 1

  • pBAR _RFP_TtrpC digestion check

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    9/14

  • pBAR _RFP_TtrpC plasmid extraction

  • RFP_TtrpC digested

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    9/16

  • pBAR _RFP_TtrpC did digestion check

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    9/17

  • pBAR_RFP_TtrpC did digestion check

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    9/18

  • Transformed pBAR_RFP_TtrpC into competent cell

  • pBAR_RFP_TtrpC did 3 in 1

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    9/20

  • Extracted plasmid pBAR_RFP_TtrpC

  • pBAR_RFP_TtrpC did digestion check

  • RFP_TtrpC digestion

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    9/21

  • pBAR_RFP_TtrpC did digestion check

  • pBAR_RFP_TtrpC did 2 in 1

  • pBARGPE1 and RFP_TtrpC digestion

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    9/22

  • Amplified RFP_TtrpC with KOD polymerase

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    9/24

  • pBAR_RFP_TtrpC did digestion check

  • Extracted plasmid pBAR_RFP_TtrpC (from 9/5 plate)

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    9/25

  • Transformed pBAR_RFP_TtrpC into competent cell

  • RFP_TtrpC digestion

  • Amplified RFP-TtrpC with KOD polymerase

  • Amplified RFP_TtrpC with KOD polymerase

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    9/26

  • pBAR_RFP_TtrpC did 2 in 1

  • Extracted plasmid pBAR_RFP_TtrpC

  • EL222 and TtrpC ran ligation PCR

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    9/28

  • pBAR_RFP_TtrpC did plasmid PCR

  • Successfully constructed the plasmid pBAR_RFP_TtrpC

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    10/5

  • ppBAR_RFP_TtrpC digestion (for ligation with Pmcl1)

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    10/8

  • pBAR _RFP_TtrpC digestion

  • Transformed pBAR_ Pmcl1_RFP_TtrpC into competent cells

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    10/9

  • pBAR_ Pmcl1_RFP_TtrpC containing cultures used in 2 in 1

  • Extracted plasmid pBAR _ Pmcl1_RFP_TtrpC

  • pBAR_ Pmcl1_RFP_TtrpC checked with restriction enzyme digestion

  • pBAR_ Pmcl1_RFP_TtrpC checked with restriction enzyme digestion

  • pBAR_ Pmcl1_RFP_TtrpC checked with restriction enzyme digestion

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    10/10

  • pBAR_ Pmcl1_RFP_TtrpC checked with restriction enzyme digestion (failure)

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