Team:DTU-Denmark/achievements
Title
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Milestones
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Aenean imperdiet - (90mins)Etiam sit amet orci eget
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Quisque rutrum. Aenean imperdiet. Etiam ultricies nisi vel augue. Curabitur ullamcorper ultricies nisi. Nam eget dui. Etiam rhoncus.
Aenean imperdiet - (~30mins)Etiam sit amet orci eget
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Aenean imperdiet - (~45mins)Medal Requirements
Bronze
✓ Register and attend
We registred for iGEM, had great and educational (sometimes frustrating) summer. We also attended the Giant jamboree.
✓ Deliverables
Yes, we met all the deliverables. Take a look around on our awesome wiki. We have registered and submitted all our parts. We have filled out the safety, judging and registry forms as required. The team is ready with the presentation and poster for the Giant Jamboree.
✓ Attribution
Even though this project was driven and conducted by the team only, we have gotten a lot of much appreciated help during this project. Everyone should of course be credited for their attributions. Please see our attribution site
✓ Part
We have registered and submitted our new part BBa_K2117003. This part encodes the Human Proinsulin peptide. The gene has been codon-optimized for the yeast Yarrowia lipolytica using a DTU-Denmark iGEM 2016 team designed codon-optimization tool that also considers illegal restriction sites.
Silver
✓ Validated Part
Our new BioBrick device BBa_K2117004 encodes the humanized Renilla reniformis green fluorescent protein (hrGFP) codon-optimized for Yarrowia lipolytica and the translation elongation factor-1α (TEF1) promoter. We have used this device to demonstrate heterologous protein expression in Y. lipolytica and experimentally validated it through confocal laser scanning microscope.
✓ Collaboration
We have helped different iGEM teams during summer: Uppsala iGEM, UNIK-cph, SDU-Denmark. For more information please see collaborations.
✓ Human Practices
We sure went beyond the lab bench during this project. Our biggest achievement was the launch of the Biosensor project, a platform for allowing over 200 high schools nationwide. We also held meet-ups for other iGEM teams, presented our project at different events and got media coverage. Please see our practices page for elaboration.
Gold
✓ Integrated Human Practices
Throughout our project we have been in close contact and collaboration with the industry. We started out searching for abundant waste streams in Denmark by contacting and visiting local factories, then we presented our idea to biotech manufacturing companies and all together the feedback made us more aware of the challenges our technology is facing and it ultimately led us to a more feasible product. Please see (link)
✓ Improve a previous part
We have improved the two BioBricks; BBa_K530001 (CrtE gene) and BBa_K530002 (CrtI gene) created by the John Hopkins iGEM team 2011. We used site directed mutagenesis to successfully remove of two illegal restriction sites and further improved the characterization. Please see the parts page for elaboration.
✓ Proof of concept
We successfully designed a functional plasmid (pSBB1A8YL) for transformation and replication in both E. coli and Y. lipolytica. pSBB1A8YL is also compatible with the BioBrick standard by assembly with our own BioBrick device BBa_K2117005. Further we showed that we could utilize the construct in Y. lipolytica to express a heterologous protein, hrGFP. Please see the proof of concept page for elaboration.
✗ Demonstrate your work
Section 3
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Section 4
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Section 5
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