Team:ASIJ Tokyo/Parts

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Parts


Anderson Promoters

  • Relative Strength: weak (0.33) BBa_J23110
    • Selected as a relatively weak promoter against which to compare the expression of the PETase construct
  • Relative Strength: moderate (0.58) BBa_J23111
    • Selected as a relatively moderately strong promoter against which to compare the expression of the PETase construct
  • Relative Strength: strong (1) BBa_J23100
    • Selected as a relatively strong promoter against which to compare the expression of the PETase construct

    • Tags

  • N-OsmY: Fusion proteins with an N-terminal osmY have been shown to successfully secrete proteins of interest out of E. Coli
  • C-Myc: This C-terminal tag would allow the PETase construct to be more easily detected by Western blot assays for expression

    • Others

  • PETase sequence: gene that coded for the PET hydrolase
  • Plasmid backbone: the pSB1C3 backbone is recommended
  • High efficiency E. Coli cells: hosted the PETase constructs

    • Parts Submitted

  • BBa_K1885001
  • BBa_K1885002
  • BBa_K1885005