Team:UFAM-UEA Brazil/Project
PROJECT
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We adopted a strategy that goes beyond of what was presented before in the competition. We aim to enhance Mer Operon expression, improving previous synthetic genetic circuits and allying them with the construction of a bioreactor for water contaminated by mercury. With our genetic construction we achieved more than 97% of mercury bioremediation in 10 hours and then we made available diverse novel Biobricks for genetic constructions related to bioremediation.
Several bacterias are potencial resistent to Hg, among those there are Serratia marcescens, Pseudomonas putida, Cupriavidus metallidurans and Entereobacter. The resistance occurs due a set of genes that direct enhance the methabolism and mercury capture. One of these proteins is the phytochelatin, which has the ability to interact with heavy metals due its cystein amino acids having strong affinity to metals.
The use of natural proteins from cell membrane to anchor heterologous proteins as cellsurface display shows great potential in the biotech field. Using this strategy many pepitites , were already anchored with several purposes such production of antibodies, biocatalizers, bioremediators and others. For heavy metal bioremediation was showed that recombinants with cellsurface display enriched with chelant proteins have better performance in adsortion of metalic ions when compared with heavy molecular engineered devices.
Nowadays there are several strategies to anchor protein in membranes. In this project we used the most abundant cell membrane protein in E. coli, the outer membrane A (OmpA) fused to a synthetic phytochelatin in order to bio-accumulate mercury.
We build the first (real) bioreactor to treat water contaminated by mercury using our chassis carrying synthetic genetic circuits we developed! We had the awesome experience working with fermentation experts and see our system working. We achieved the ultimate proof of concept and it was the first step to have this synbio system working out there in the real world.
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