Primers used
Primer | Sequence |
---|---|
oMEMO7624_Fw_Verification_pSB1C3 | CCACCTGACGTCTAAGAAAC |
oMEMO7625_Rv_Verification_pSB1C3 | GTATTACCGCCTTTGAGTGA |
oMEMO7626_inaX?_fw | ATGATCGTTGAAAAGATTTTGGCATTGCGTAC |
oMEMO7627_inaX?_rv | TCAGACATCGCGCAGTTCCTCGCCAGTGCC |
oMEMO7628_BsaI-inaZ_fw | ACGTGGTCTCTGATGAATCTCGACAAGGCGTTG |
oMEMO7629_BsaI-inaZ_rv* | ACGTGGTCTCTAGTACTACTTTACCTCTATCCAGTC |
oMEMO7643_Rv_BB_strong | AGGACTGAGCTAGCTGTCAACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAGCG |
oMEMO7644_Rv_BB_weak | AGGACTGAGCTAGCCGTAAACTCTAGAAGCGGCCGCGAATTCCAGAAATCATCCTTAGCG |
oMEMO7645_Fw_BB | TGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAG |
oMEMO7658_inaZ_GGfix_2_Fw | AGTAGGTCTCAGTAACGAAACCGCTGGCAACCACAGTGATC |
oMEMO7659_inaZ_GGfix_3_Fw | TAACGGTCTCAGGCGGGTGACCAAAGCAGATTGACCGCTG |
oMEMO7661_GFP_Fw-ATCT | AGTCGGTCTCAATCTAGTAAAGGAGAAGAACTTTTCACTGGAG |
oMEMO7662_GFP_Fw-GATG | AGTCGGTCTCAGATGAGTAAAGGAGAAGAACTTTTCACTGGAG |
oMEMO7663_GFP_Rv-ATCT | AGCTGGTCTCAAGATCCGGTGCTGCCTTTGTAGAGCTCATCCATGCCATG |
oMEMO7664_GFP_Rv-TACT | AGCTGGTCTCAAGTATTATTTGTAGAGCTCATCCATGCCATG |
oMEMO7665_INP_RC_Fw | AGTCGGTCTCAGATGCCGCAGAATGTGCAGAC |
oMEMO7666_INP_RC_Rv | AGCTGGTCTCAAGATCCGGTGCTGCCCTTTACCTCTATCCAGTCATCGTCCTCGTC |
oMEMO7667_Lpp_Fw | AGTCGGTCTCAGATGAAAGCTACTAAACTGGTACTGG |
oMEMO7668_Strep_Rv | AGTCGGTCTCAAGTATTATTAATGGTGGTGATGGTGATGGGA |
oMEMO7669_FW_BB_LC | TGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGGAGTCACTAAGGGTTAG |
oMEMO7670_RV_BB_weak_LC | AGGACTGAGCTAGCCGTAAACTCTAGAAGCGGCCGCGAATTCGAGTCACTAAGGGCTAAC |
oMEMO7671_RV_insert | AGTATATAAACGCAGAAAGGCC |
oMEMO7672_FW_Insert_weak | TTTACGGCTAGCTCAGTCCTAG |
oMEMO7673_inaZ_GGfix_1_Rv | CAATTAGATCACTGTGGTTGCCAG |
oMEMO7674_inaZ_GGfix_2_Rv | TCTTACCAGCGGTCAATCTGCTTTGGTC |
oMEMO7675_FW_BB_pSB3K3 | TGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAACGGGCAAG |
oMEMO7676_BB_mSA2_Fw | GCTGCAGTCCGGCAAAAAAGGGCAAG |
oMEMO7677_BB_mSA2_Rv | CCGCGAATTCCAGAAATCATCCTTAGCG |
oMEMO7714_bb-pSC101_fw | CCTAGGACTGAGCTAGCTGTCAATAGGGCAGCAGATAGGGACGAC |
oMEMO7715_bb-pSC101_rv | GTGGGCCTTTCTGCGTTTATATACTCTTTTATTGGAGAGGTGGACAAG |
oMEMO7722_BB_GFP_Fw | ACGTGGTCTCATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAAGGGCAAGGTG |
oMEMO7723_BB_GFP_Rv | AGTCGGTCTCACATCTAGAAGCGGCCGCGAATTCCAGAAATCATCC |