Prokaryotic Version of Signal Filter:
Transcriptional regulation:
| Part Number | Name | Description |
| BBa_K2036000 | RBS-CII | A protein in bacteriophage λ operon which can activate promoter RE. |
| BBa_K2036001 | CIII | A protein in bacteriophage λ operon which can free CII from its inhibitor, FtsH. |
| BBa_K2036006 | RBS-CIII | A protein in bacteriophage λ operon which can free CII from its inhibitor, FtsH. |
Note: We didn’t submit the single parts of pRE, pRM and pR because they are too small to construct, but we have constructed and submitted the composite type of them, using GFP as the report gene in the characterization pathways.
Protein&Promoter Interaction:
| Part Number | Name | Description |
| BBa_K2036007 | RBS-CII-RBS-CII | A protein in bacteriophage λ operon which can activate promoter RE. We use tandem to amplify its function. |
| BBa_K2036012 | RBS-CII-TT | A protein in bacteriophage λ operon which can activate promoter RE. We use tandem to amplify its function. |
| BBa_K2036011 | pRE-GFP-LVAssrAtag | The report part of the characterization pathway and it’s used to characterize promoter RE. |
| BBa_K2036032 | CII-TT-pRE-RBS-GFP-LVAssrAtag | To characterize function of CII. |
Protein&protein interaction:
| Part Number | Name | Description |
| BBa_K2036007 | RBS-CII-RBS-CII | A protein in bacteriophage λ operon which can activate promoter RE. We use tandem to amplify its function. |
| BBa_K2036008 | RBS-CIII-RBS-CIII | A protein in bacteriophage λ operon which can free CII from its inhibitor, FtsH. We use tandem to amplify its function. |
| BBa_K2036013 | RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag | To characterize CII’s function. |
Note: CIII and CII circuit above shares the same assembly composition of RE-RBS-GFP-LVAssrAtag with CII and pRE characterization circuit so the part is not listed repeatedly.
Tri-stable function:
| Part Number | Name | Description |
| BBa_K2036018 | pRE-RBS-Cro-RBS-CII-TT-ptrp | Used to construct the circuit. A part of the positive feedback system. |
| BBa_K2036019 | RFP-LVAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag | The report part of the circuit.RFP is used as gene of interest 1 and GFP as gene of interest 2. |
| BBa_K2036020 | RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LVAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag | Used to construct the circuit. |
Eukaryotic version of Signal Filter:
Functional protein and promoter:
| Part Number | Name | Description |
| BBa_K2036005 | PP2CA | Clade A protein phosphatases type 2C which can dephosphorylate ABF2. |
| BBa_K2036004 | ABF2 | A leucine zipper transcription factor which can activate pRD29A. |
| BBa_K2036003 | SnRK2.2 | Sucrose Nonfermenting1 Related Subfamily2 (SnRK2s) protein kinase which can phosphorylate ABF2. |
| BBa_K2036002 | prd29A | A promoter in the Abscisic Acid signaling network which can be activated by ABF2. |
Bi-stable-function
| Part Number | Name | Description |
| BBa_K2036025 | Kozak-Snrk2.2-TTADH1-pCyc | Used to construct the circuit. |
| BBa_K2036029 | Kozak-ABF2-TTADH1-prd29A-Kozak-GFP-LVAssrAtag | The report part of the circuit.GFP is used as gene of interest. |
| BBa_K2036031 | PP2CA-TTADH1-pCyc | Used to construct the circuit. |
Application of Signal Filter:
| Part Number | Name | Description |
| BBa_K2036021 | placm-pRE-RBS-Cro-RBS-CII-TT | Used to construct the circuit. A part of the positive feedback system. placm can response to lactose signal. |
| BBa_K2036022 | patp2-RBS-CI-TT-pR-RBS-CIII | Used to construct the circuit. Patp2 can response to base signal. |
| BBa_K2036023 | RBS-iLDH-TT-pRM-RBS-beta-galactosidase | The report part of the application circuit.iLDH is used as gene of interest 1 and beta-galactosidase the gene of interest 2. |
| BBa_K2036028 | placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-ompA-iLDH-TT-pRM-RBS-beta-galactosidase | The whole circuit of the application. |
