Team:NYMU-Taipei/Notebook-Lab Book-TtrpC

TtrpC

TtrpC


[Collapse all] | [Expand all]

7/22

  • Received plasmid pAN7-1 from Kaohsiung District Agricultural Research and Extension Station

  • pAN7-1 transformation

  • pAN7-1 did 2 in 1

    • [Collapse]

    7/23

  • pAN7-1 plasmid extraction

    • [Collapse]

    7/24

  • pAN7-1 amplified with plasmid PCR (primer: minPgpdA)

  • pAN7-1 extracted and transformed cultures cryopreserved

    • [Collapse]

    7/29

  • VP-TR amplified with KOD polymerase PCR (failed, contaminated NC)

    • [Collapse]

    7/31

  • VP-TR trouble shooting

  • VP-TR PCR amplification(correct)

    • Target sequence: TtrpC :791 base pairs

    [Collapse]

    8/13

  • TtrpC PCR amplification for EL222_RFP

    • [Collapse]

    8/15

  • TtrpC and pVP-EL222 PCR amplificaiton

    • [Collapse]

    8/16

  • TtrpC digestion

    • [Collapse]

    8/23

  • TtrpC PCR amplification

  • TtrpC digestion

    • [Collapse]

    8/26

  • TtrpC checked with plasmid PCR (with remixed pVP-EL222 primers)

    • [Collapse]

    8/27

  • TtrpC KOD PCR amplification

    • [Collapse]

    9/11

  • TtrpC amplified with KOD polymerase

  • TtrpC amplified with KOD polymerase

    • [Collapse]

    9/19

  • Amplified TtrpC form plasmid pAN7-1

    • [Collapse]

    10/1

  • TtrpC digestion (for the ligation with pBARGPE1)

    • [Collapse]