Team:NYMU-Taipei/Notebook-Lab Book-TtrpC

TtrpC

TtrpC


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7/22

  • Received plasmid pAN7-1 from Kaohsiung District Agricultural Research and Extension Station

  • Transformed plasmid pAN7-1 into competent cells

  • plasmid pAN7-1 containing cultures used in 2 in 1

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    7/23

  • pAN7-1 plasmid extraction

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    7/24

  • pAN7-1 amplified with plasmid PCR (primer: minPgpdA)

  • pAN7-1 extracted and transformed cultures cryopreserved

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    7/29

  • VP-TR amplified with KOD polymerase PCR (failed, contaminated NC)

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    7/31

  • VP-TR trouble shooting

  • VP-TR PCR amplification(correct)

    • Target sequence: TtrpC :791 base pairs

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    8/13

  • TtrpC PCR amplification for the ligation with EL222_RFP

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    8/15

  • TtrpC and pVP-EL222 PCR amplificaiton

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    8/16

  • TtrpC digestion

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    8/23

  • TtrpC amplified with KOD polymerase

  • TtrpC digestion

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    8/26

  • TtrpC checked with plasmid PCR (with remixed pVP-EL222 primers)

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    8/27

  • TtrpC KOD PCR amplification

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    9/11

  • TtrpC amplified with KOD polymerase

  • TtrpC amplified with KOD polymerase

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    9/19

  • Amplified TtrpC form plasmid pAN7-1

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    10/1

  • TtrpC digestion (for the ligation with pBARGPE1)

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