Team:NYMU-Taipei/Notebook-Lab Book-pVP-EL222P

pBARGPE1

pBARGPE1


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7/4

  • Received plasmid pVP-EL222 from University of Texas Southwestern

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    7/13

  • pVP-EL222 resuspended (sample from University of Texas Southwestern)

  • pVP-EL222 transformed into competent cells

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    7/14

  • pVP-EL222 did 3 in 1

  • pVP-EL222 extracted from transformed cultures and checked with restriction digestion (EcoRI andXhoI)

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    7/15

  • pVP-EL222 plasmid stored and transformed cultures cryopreserved

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    7/29

  • pVP-EL222 amplified with plasmid PCR (contaminated NC)

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    7/30

  • pVP-EL222 checked with plasmid PCR with brand new reagents (success, no contamination detected)

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    7/31

  • pVP-EL222 amplified with plasmid PCR

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    8/1

  • pVP-EL222 digested with SpeI and BamHI)

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    8/15

  • pVP-EL222 amplified with PCR

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    8/20

  • pVP-EL222 amplified with PCR than digested

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    8/25

  • pVP-EL222 checked separately with plasmid PCR with redesigned primers

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    8/26

  • pVP-EL222 checked with plasmid PCR due to multiple failed ligations

  • pVP-EL222 checked with restriction digestion due to multiple failed ligations

  • pVP-EL222 checked with Taq polymerase plasmid PCR with remixed primers

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    8/27

  • pVP-EL222 checked with KOD polymerase plasmid PCR with remixed primers

  • pVP-EL222 amplified with PCR (use for ligation with both pBAR_PgpdA and PMCL1)

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    9/7

  • pVP-EL222 amplified with PCR (use for ligation with both pBAR_PgpdA and PMCL1)

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    9/8

  • pVP-EL222 amplified with PCR (use for ligation with both pBAR_PgpdA and PMCL1)

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