pVP-EL222P
pVP-EL222P/h1>
Received plasmid pVP-EL222 from University of Texas Southwestern
pVP-EL222 resuspended (sample from University of Texas Southwestern)
pVP-EL222 transformed into competent cells
pVP-EL222 did 3 in 1
pVP-EL222 extracted from transformed cultures and checked with restriction digestion (EcoRI andXhoI)
pVP-EL222 plasmid stored and transformed cultures cryopreserved
pVP-EL222 amplified with plasmid PCR (contaminated NC)
pVP-EL222 checked with plasmid PCR with brand new reagents (success, no contamination detected)
pVP-EL222 amplified with plasmid PCR
pVP-EL222 digested with SpeI and BamHI)
pVP-EL222 amplified with PCR
pVP-EL222 amplified with PCR than digested
pVP-EL222 checked separately with plasmid PCR with redesigned primers
pVP-EL222 checked with plasmid PCR due to multiple failed ligations
pVP-EL222 checked with restriction digestion due to multiple failed ligations
pVP-EL222 checked with Taq polymerase plasmid PCR with remixed primers
pVP-EL222 checked with KOD polymerase plasmid PCR with remixed primers
pVP-EL222 amplified with PCR (use for ligation with both pBAR_PgpdA and PMcl1)
pVP-EL222 amplified with PCR (use for ligation with both pBAR_PgpdA and PMcl1)
pVP-EL222 amplified with PCR (use for ligation with both pBAR_PgpdA and PMcl1)