Team:SCUT-China A/Achievement part
Type
Function
Length
dszA (a monooxygenase gene )
coding
convert DBTsulfone (DBTO2) to 2-(2-hydroxybiphenyl) sulfinate (HBPS) during the 4S pathway
1383 bp
dszB (a desulfinase gene
coding
convert 2-(2-hydroxybiphenyl) sulfinate (HBPS) to 2-hydroxybiphenyl (2HBP) during the 4S pathway
1117 bp
dszC (a monooxygenase gene )
coding
not only convert dibenzothiophene (DBT) to DBT-sulfoxide (DBTO) but also convert DBT-sulfoxide (DBTO) to DBTsulfone (DBTO2)during the 4S pathway
1274 bp
dszD (a flavinreductase)
coding
three successive oxidation steps require reducing equivalents (FMNH2) supplied by a flavinreductase (DszD) during the 4S pathway
599 bp
dszA (a monooxygenase gene ), E.coli-optimized
coding
convert DBTsulfone (DBTO2) to 2-(2-hydroxybiphenyl) sulfinate (HBPS) during the 4S pathway
1383 bp
dszB (a desulfinase gene), E.coli-optimized
coding
convert 2-(2-hydroxybiphenyl) sulfinate (HBPS) to 2-hydroxybiphenyl (2HBP) during the 4S pathway
1170 bp
dszC (a monooxygenase gene ), E.coli-optimized
coding
not only convert dibenzothiophene (DBT) to DBT-sulfoxide (DBTO) but also convert DBT-sulfoxide (DBTO) to DBTsulfone (DBTO2)during the 4S pathway
1274 bp
dszBCAD with T7 promoter
coding
dszBCAD genes with T7 promoter which can convert dibenzothiophene (DBT) to 2-hydroxybiphenyl (2HBP)
4537 bp
T7 RNA polymerase induced by IPTG
coding
T7 RNA polymerase will be expressed with IPTG added.
4552 bp
dszBCAD with Lac promoter
coding
dszBCAD genes with Lac promoter which can convert dibenzothiophene (DBT) to 2-hydroxybiphenyl (2HBP)
5859 bp
dszBAC with tac promoter and dszD with lac promoter
coding
dszBAC genes with tac promoter and dszD gene with lac promoter which can convert dibenzothiophene (DBT) to 2-hydroxybiphenyl (2HBP)
6052 bp
dszBCAD with T7 promoter induced by IPTG
composite
When IPTG added, dszBCAD genes with T7 promoter will be expressed greatly which can convert dibenzothiophene (DBT) to 2-hydroxybiphenyl (2HBP)
9097 bp
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South China University of tecnology
GuangZhou,China
Achievement parts
