Team:NYMU-Taipei/Notebook-Lab Book-pBAR Pmcl1 EL222 TtrpC

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8/16

  • pBAR_EL222_TtrpC transformed into competent cells

  • pBAR_EL222_TtrpC containing cultures used 2 in 1

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    8/17

  • pBAR_EL222_TtrpC extracted and attempted to amplify with plasmid PCR (we did not amplify the target sequence; hence, we tried to ligate again)

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    8/18

  • pBAR_EL222_TtrpC transformed into competent cells

  • pBAR_EL222_TtrpC containing cultures used in 3 in 1

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    8/19

  • C120_minimal PgpdA digested for the ligation with RFP

  • pBAR_EL222_TtrpC extracted from transformed cultures, checked with restriction digestion and plasmid PCR (failure)

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    8/28

  • pVP-EL222 and TtrpC PCR ran ligation PCR (NC had been contaminated)

  • pVP-EL222 and TtrpC PCR ran ligation PCR (used remixed primer)

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    8/29

  • pVP-EL222 and TtrpC ran ligation PCR (the concentration was not high enough after gel extraction)

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    8/31

  • pVP-EL222 and TtrpC PCR ran ligation PCR

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    9/1

  • pVP-EL222 and TtrpC ran ligation PCR

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    9/2

  • EL222-TtrpC digestion

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    9/3

  • pBAR_EL222_TtrpC transformed into competent cells

  • pBAR_EL222_TtrpC containing cultures used in 3 in 1

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    9/5

  • pBAR_EL222_TtrpC checked with restriction digestion

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    9/6

  • EL222-TtrpC digestion

  • pBAR_EL222_TtrpC checked with restriction digestion

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    9/7

  • pBAR_EL222 checked with plasmid PCR

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    9/16

  • pBAR_EL222_TtrpC checked with plasmid PCR

  • pBAR_EL222_TtrpC checked with plasmid PCR

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    9/17

  • pBAR_EL222_TtrpC plasmid stored and transformed cultures cryopreserved

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