Team:NYMU-Taipei/Notebook-Lab Book-pBAR PgpdA EL222 TtrpC

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8/5

  • pBAR_PgpdA transformed into competent cells

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    8/6

  • pBAR_PgpdA containing cultures used in 2 in 1

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    8/7

  • pBAR_PgpdA extracted and checked with plasmid PCR and restriction digestion

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    8/14

  • pBAR_PgpdA plasmid stored and transformed cultures cryopreserved

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    8/18

  • plasmid pBAR_PgpdA digested for ligation with pVP-EL222 and TtrpC

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    8/19

  • pBAR_PgpdA_EL222_TtrpC transformed into competent cells

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    8/21

  • pBAR_PgpdA_EL222_TtrpC extracted and checked with restriction digestion (failure)

  • pBAR_PgpdA_EL222_TtrpC transformed into competent cells

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    8/23

  • pBAR_PgpdA_EL222_TtrpC containing cultures used in 3 in 1

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    8/24

  • pBAR_PgpdA_EL222_TtrpC checked with restriction digestion

  • pBAR_PgpdA_EL222_TtrpC checked with plasmid PCR

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    8/28

  • pVP-EL222 and TtrpC ran ligation PCR (NC had been contaminated)

  • pVP-EL222 and TtrpC ran ligation PCR (with remixed primer)

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    8/29

  • pVP-EL222 and TtrpC ran ligation PCR (the concentration was not high enough after gel extraction)

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    8/31

  • EL222 and TtrpC ran ligation PCR

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    9/1

  • EL222 and TtrpC ran ligation PCR

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    9/2

  • EL222-TtrpC checked with restriction digestion

  • pBAR_PgpdA digested for ligation with EL222_TtrpC

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    9/3

  • pBAR_PgpdA digested for the ligation with EL222_Ttrp

  • pBAR_PgpdA_EL222_TtrpC transformed into competent cells

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    9/4

  • pBAR_PgpdA_EL222_TtrpC containing cultures used in 3 in 1

  • pBAR_PgpdA_EL222_TtrpC extracted from transformed cultures

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    9/5

  • pBAR_PgpdA_EL222_TtrpC checked with plasmid PCR

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    9/6

  • pBAR_PgpdA_EL222_TtrpC checked with restriction digestion (failure)

  • pBAR_PgpdA_EL222_TtrpC checked with restriction digestion (we changed enzymes)

  • EL222-TtrpC digested with restriction digestion

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    9/9

  • pBAR_PgpdA_EL222_TtrpC checked with restriction digestion

  • pBAR_PgpdA_EL222_TtrpC transformed into competent cells (no E.coli colonies appeared on our first plate )

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    9/10

  • pBAR_PgpdA_EL222_TtrpC transformed into competent cells

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    9/11

  • pBAR_PgpdA_EL222_TtrpC containing culture used in 3 in 1

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    9/12

  • pBAR_PgpdA_EL222_TtrpC checked with restriction digestion

  • pBAR_PgpdA_EL222_TtrpC checked with plasmid PCR

  • Successfully constructed the plasmid pBAR_PgpdA_EL222_TtrpC

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    9/22

  • pBAR_PgpdA_EL222_TtrpC digested for transforming into Metarhizium anisopliae

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