Team:Saint Rose School B/Notebook



Notebook

Journal:

  • June


  • Thursday 2nd:
  • Scientific fair, Academia de Emprendimiento.


  • Saturday 25th:
  • We search for lignin degradation enzymes.


  • Wednesday 29th:
  • We investigate for more of these enzymes.

  • July


  • Friday 1st:
  • We create our GMO.


  • Saturday 2nd:
  • We did cultivate bacteria with LuxI, LuxR and PLux.


  • Wednesday 6th:
  • We extract the plasmids of LuxR, LuxI and PLux from the E.coli bacteria and check it (electrophoresis).


  • Friday 8th:
  • We extract LiP and Lac from the plates and then we transform it.


  • Wednesday 13th:
  • We made again the cultivate with the plasmid extraction of LuxR, LuxI and PLux.
  • We cultivate LiP and Lac.


  • Friday 15th:
  • Double digestion of LuxR, LuxI and PLux.
  • Check the electrophoresis.



  • Friday 22nd:
  • We found the space where the genes (RFP and CFP) are, and we extract it from the plates and we transform it.



  • Saturday 23rd:
  • We extract the plasmid of LiP and Lac from the cultive, and then we did electrophoresis to check the size.



  • Friday 29th:
  • We cultiveted bacteria of RFP and CFP, and then electrophoresis to check the size of the plasmids.

  • August


  • Wednesday 3rd:
  • We extract the plasmid of RFP y CFP.
  • Presentation of groups progress.


  • Friday 5th:
  • We did double digestion of LiP and Lac, then we did electrophoresis to check the size.
  • Check the information.


  • Saturday 6th:
  • We dedicated to read papers.
  • We do some investigation and search.
  • We check the result of the plasmidial extraction of CFP and RFP.


  • Wednesday 10th:
  • Check of information.
  • Add ClbR an Cbh1 to our modeling design.
  • We helped to others school in the check of extractions.
  • Reading of papers.


  • Friday 12th:
  • Question to the team.
  • Little rehearsal team.
  • Investigation of ClbR an Cbh1 function.


  • Saturday 13th:
  • We practice genes ligation with others schools.


  • Saturday 20th:
  • Lab work (Valentina, Bastian e Isabela).
  • Helped to the others groups in the lab (Natalia, Macaren, Karen and Maxi).
  • We work on the poster information.


  • Wednesday 24th:
  • Wiki works.
  • Trabajo con calcio competentes.


  • Friday 26th:
  • We work on the wiki information.
  • We search for the coding sequence of ClbR and Cbh1.


  • Saturday 27th:
  • We take photos for our wiki.


  • Wednesday 31st:
  • Help other schools in lab.

  • September


  • Saturday 3rd:
  • Wiki works.
  • Design class for website (introduction).


  • Wednesday 7th:
  • Changes in the modeling design (final design).
  • Wiki works.


  • Saturday 10th:
  • Production of news modeling parts.
  • Wiki works.
  • Design class to website.


  • Wednesday 14th:
  • Last website class.
  • Wiki works.
  • Reading of papers.


  • Friday 16th:
  • Lab work (Karen, Natalia, Maximiliano y Valentina).
  • Wiki (Isabella)
  • Reading of papers (Bastian y Maca)


  • Saturday 17th:
  • Wiki
  • Little group rehearsal.


  • Wednesday 21st:
  • Wiki
  • Progress of presentations.


  • Saturday 24th:
  • Wiki
  • Plane our iGEM presentation.


  • Wednesday 28th:
  • Speak about our project.


  • Friday 30th:
  • Information and design of Wiki.


  • Octuber


  • Saturday 1st:
  • Wiki Design.
  • Poster Design.


  • Wednesday 5th:
  • Wiki
  • Meeting with the Doctorate.


  • Friday 7th:
  • Preparation to the fair.


  • Saturday 8th:
  • Science fair in MIM (Chilean museum).


  • Monday 10th:
  • Wiki


  • Wednesday 12th:
  • Wiki
  • Work with specialize in biotechnology.


  • Wednesday 14th:
  • Final design poster.
  • Last details of experimental biological part.


  • Saturday 15th:
  • Final information and design to our wiki.
  • Doctorate presentation.


  • Wednesday 19th:
  • iGEM Presentation Practice.