Team:Paris Saclay/Notebook/July/28
Contents
Thursday 28th July
Lab work
Visualization
Low Fidelity DreamTaqPCR of DH5alpha transformed with pPS16_005 and pPS16_009
By Laetitia
PCR was performed on 6 clones for DH5a|pPS16_005 and 6 clones for DH5a|pPS16_009. Thus, the PCR mix was done for 12 tubes following the usual protocol. Each clone was taken off from the petri dish (27/07) soaked in a PCR mix and finally re-plated on a Petri dish (LB+AMP+X-Gal+IPTG. PCR was done with a Tm at 57°C. Each PCR Product was put to migrate on an agarose gel.
