Team:Bielefeld-CeBiTec/Notebook/Mutation

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Lab Notebook Mutation

Lab-Notebook 06.08.2016

PCR product for assembly of RFP expression template

Amplification of RBS-RFP-Terminator insert from pSB1C3:E1010

polymerase	Q5
for-Primer	CH22 RBS_RFP_Ter_for
rev-Primer	CH23 RBS_RFP_Ter_rev
template	pSB1C3::E1010
Tm	59 °C
elongation time	24 sec

Amplification of the pSB1C3-backbone with addition of different Anderson promoters

polymerase	Q5
for-Primer	CH24 C3BB_Ter_for
rev-Primer	CH25/26/27 C3BB_And_X_rev (X=01/047/086)
template	pSB1C3::E1010
Tm	64 °C
elongation time	1:07 min:sec