Team:CU-Boulder/Notebook
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Notebook
Week 1: 5/8/16-5/14/16
Successful microscopy has confirmed the viability of EutS and EutC-eGFP in E.Coli, but the laser used to excite eGFP may also cause conformational change of azo-benzene. Instead Neptune, another light activated protein that is excited at a much longer wavelength, will be used to visualize the formation and destruction of EutS microcompartments. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance. Instead Neptune, another light activated protein that is excited at a much longer wavelength, will be used to visualize the formation and destruction of EutS microcompartments. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance.Instead Neptune, another light activated protein that is excited at a much longer wavelength, will be used to visualize the formation and destruction of EutS microcompartments. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance.
Week 2: 5/15/16-5/21/16
BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb
Week 3: 5/22/16-5/28/16
BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb
Week 4: 5/29/16-6/4/16
BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb
Week 5: 6/5/16-6/11/16
BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb
Week 6: 6/12/16-6/18/16
BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb
Week 7: 6/19/16-6/25/16
BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb
Week 8: 6/26/16-7/2/16
BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb
Week 9: 7/3/16-7/9/16
BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb
Week 10
BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb
Week 11: 7/10/16-7/16/16
BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb
Week 12: 7/17/16-7/23/16
BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb
Week 13: 7/24/16-7/30/16
BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb
Week 14: 7/31/16-8/6/16
Successful microscopy has confirmed the viability of EutS and EutC-eGFP in E.Coli, but the laser used to excite eGFP may also cause conformational change of azo-benzene. Instead Neptune, another light activated protein that is excited at a much longer wavelength, will be used to visualize the formation and destruction of EutS microcompartments. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance. Instead Neptune, another light activated protein that is excited at a much longer wavelength, will be used to visualize the formation and destruction of EutS microcompartments. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance.Instead Neptune, another light activated protein that is excited at a much longer wavelength, will be used to visualize the formation and destruction of EutS microcompartments. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance.
Week 15: 8/7/16-8/13/16
BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb
Week 16
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Week 17: 8/14/16-8/20/16
BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb
Week 18
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Week 19
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Week 20
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Week 21
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Week 22
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Week 23
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Week 24
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Week 25
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Week 26
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Week 27
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