Team:Paris Bettencourt/Notebook/Indigo


Week 27th June - 3rd July

Week 4th - 10th July

We received the E. coli BL21 and BL21(DE3) - same background, but BL21 does not express the T7 polimerase and BL21(DE3) does.
Highlights
Transformation efficiency: 1–5 x 107 cfu/μg pUC19 DNA
Routine non-T7 expression
Deficient in proteases Lon and OmpT
Resistant to phage T1 (fhuA2)
B Strain
Free of animal products
Genotype
fhuA2 [lon] ompT gal [dcm] ΔhsdS

Week 11th - 17th July

Week 18th -24th July

Week 25th -31th July


Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
+33 1 44 41 25 22/25
igem2016parisbettencourt@gmail.com
2016.igem.org