Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Wednesday 17th August 1.1 Lab work 1.1.1 Visualization 1.1.1.1 Extraction of pPS16003, pPS16004 and GFP1-9_PSB1C3 (clones 2 and 3) 1.1.1.2 Purification of gBlocks 2, 3 and 4 1.1.2 Visualization 1.1.2.1 Cloning with pJET the gBlock 1.2, FRB, FKBP and SgNM Wednesday 17th August Lab work Visualization Extraction of pPS16003, pPS16004 and GFP1-9_PSB1C3 (clones 2 and 3) By Charlène Clones 2 and 3 of GFP1-9_PSB1C3, and pPS16003, pPS16004 were extracted with the Plasmid MiniPrep kit. The extracts were put to migrated on a 0.8%agarose gel with BET. Purification of gBlocks 2, 3 and 4 By Terrence The purification was carried out following the usual protocol. Visualization Cloning with pJET the gBlock 1.2, FRB, FKBP and SgNM By Léa and Naiane The cloning was carried out using a new protocol which uses pJET as cloning vector. A heat shock transformation was made on the cloning samples using the following protocol
By Charlène
Clones 2 and 3 of GFP1-9_PSB1C3, and pPS16003, pPS16004 were extracted with the Plasmid MiniPrep kit.
The extracts were put to migrated on a 0.8%agarose gel with BET.
By Terrence
The purification was carried out following the usual protocol.
By Léa and Naiane
The cloning was carried out using a new protocol which uses pJET as cloning vector.
A heat shock transformation was made on the cloning samples using the following protocol
