Team:BGIC China/protocols/T4-ligation

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T4连接
30ul T4连接体系：
 
10x T4 ligase buffer --- 3ul
Vector --- 30ng
DNA fragment(s) --- 30-50ng
T4 ligase --- 0.5ul
ddH2O --- 补至30ul
 
Vortex混匀后4℃冰箱静置5min
16℃连接1h
 
 
耗材来源及信息
 
 
您可点击回复以对以上信息进行更正/添加
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Team:BGIC China/protocols/T4-ligation

T4连接

30ul T4连接体系： 10x T4 ligase buffer --- 3ulVector --- 30ngDNA fragment(s) --- 30-50ngT4 ligase --- 0.5ulddH2O --- 补至30ul Vortex混匀后4℃冰箱静置5min16℃连接1h

耗材来源及信息

您可点击回复以对以上信息进行更正/添加

30ul T4连接体系：

10x T4 ligase buffer --- 3ul
Vector --- 30ng
DNA fragment(s) --- 30-50ng
T4 ligase --- 0.5ul
ddH2O --- 补至30ul

Vortex混匀后4℃冰箱静置5min
16℃连接1h