Team:BGIC China/protocols/q5-pcr

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  • Q5 mix 配方

    50ul体系：

    5X PCR buffer --- 10ul

    high CG enhancer --- 4ul

    dNTPs --- 4ul

    Primer 1 --- 2ul

    Primer 2 --- 2ul

    Template --- 0.2ul

    Q5 polymerase --- 0.5ul

    ddH2O --- 31.5ul

  • Q5 程序

    1. 98℃ 30s

    2. 98℃ 10s

    3. 55℃ 15s

    4. 72℃ 30s/kb

    2到4步30个循环

    5. 72℃ 1min

    6. 4℃ infinite

  • 耗材来源及信息