Difference between revisions of "Team:IISc Bangalore/Design"
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| + | <p>While the biotechnology industry has progressed greatly, the benefits remain inaccessible to large populations, due to factors such as R&D, infrastructure costs, etc. | ||
| + | Our project develops tools and methodologies to broadly help with the last issue – the infrastructure costs, especially for biotech entrepreneurship. To achieve this, we focussed on genetically modifying E coli in order to automate as many processes in bioreactor operation and post-fermentation processing as possible, and came up with: | ||
| + | (i) Over-express proteins under regulation of an internal quorum sensing system instead of external induction, to save on costs and eliminate necessity to monitor cell density. | ||
| + | (ii) Achieve inducible and reversible inter-cell adhesion to allow for aggregation and separation of cell from media dependent on nutrient content in the media, to save costs on centrifugation. | ||
| + | (iii) A large fraction of the cells are removed, the cells deaggregated and fresh media added, returning the system to its initial state. | ||
| + | </p> | ||
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Revision as of 22:05, 14 October 2016
While the biotechnology industry has progressed greatly, the benefits remain inaccessible to large populations, due to factors such as R&D, infrastructure costs, etc. Our project develops tools and methodologies to broadly help with the last issue – the infrastructure costs, especially for biotech entrepreneurship. To achieve this, we focussed on genetically modifying E coli in order to automate as many processes in bioreactor operation and post-fermentation processing as possible, and came up with: (i) Over-express proteins under regulation of an internal quorum sensing system instead of external induction, to save on costs and eliminate necessity to monitor cell density. (ii) Achieve inducible and reversible inter-cell adhesion to allow for aggregation and separation of cell from media dependent on nutrient content in the media, to save costs on centrifugation. (iii) A large fraction of the cells are removed, the cells deaggregated and fresh media added, returning the system to its initial state.
