Difference between revisions of "Team:Paris Saclay/Notebook/October/10"

(Created page with "{{Team:Paris_Saclay/notebook_header}} = Monday 10<sup>th</sup> October= ==Lab work== ===BioBrick K2039000 and K2039001 characterization=== ====Protein extraction of FRB - GF...")
 
(Protein extraction of FRB - GFP 11 in pSB1C3 (pPS16_019) clone 4, FKBP - GFP 10 in pSB1C3 (pPS16_018) clone 6, GFP 1.9 in pUC19 (pPS16_009) clone 1 and PhB1040 strain 594)
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''By Maxence''
 
''By Maxence''
  
In order to perform a Western Blot, the proteins were extracted from clones (pPS16_018 clone 6, pPS16_019 clone 4, pPS16_009 clone 1 and PhB1040 strain 594) put on liquide culture the 7th October. For that purpose,  
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In order to perform a Western Blot, the proteins were extracted from clones (pPS16_018 clone 6, pPS16_019 clone 4, pPS16_009 clone 1 and PhB1040 strain 594) put on liquide culture the 7th October. For that purpose, the [[Team:Paris_Saclay/Experiments#Protein_extraction_from_bacterial_liquid_culture_for_Western_Blot|protein extraction protocol]] was used:
 
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{| class="wikitable"
 +
|-
 +
!Sample
 +
!OD (1 ml)
 +
!Volume of lysis buffer
 +
|-
 +
|FRB - GFP 11 in pSB1C3 (pPS16_019)
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|5.1
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|127.5 μl
 +
|-
 +
|FKBP - GFP 10 in pSB1C3 (pPS16_018)
 +
|5.2
 +
|130 μl
 +
|-
 +
|GFP 1.9 in pUC19 (pPS16_009)
 +
|5.3
 +
|132.5 μl
 +
|-
 +
|PhB1040 strain 594
 +
|2.8
 +
|70 μl
 +
|-
 +
|}
  
  

Revision as of 13:14, 12 October 2016

Monday 10th October

Lab work

BioBrick K2039000 and K2039001 characterization

Protein extraction of FRB - GFP 11 in pSB1C3 (pPS16_019) clone 4, FKBP - GFP 10 in pSB1C3 (pPS16_018) clone 6, GFP 1.9 in pUC19 (pPS16_009) clone 1 and PhB1040 strain 594

By Maxence

In order to perform a Western Blot, the proteins were extracted from clones (pPS16_018 clone 6, pPS16_019 clone 4, pPS16_009 clone 1 and PhB1040 strain 594) put on liquide culture the 7th October. For that purpose, the protein extraction protocol was used:

Sample OD (1 ml) Volume of lysis buffer
FRB - GFP 11 in pSB1C3 (pPS16_019) 5.1 127.5 μl
FKBP - GFP 10 in pSB1C3 (pPS16_018) 5.2 130 μl
GFP 1.9 in pUC19 (pPS16_009) 5.3 132.5 μl
PhB1040 strain 594 2.8 70 μl