(Created page with "{{Team:Paris_Saclay/notebook_header}} = Monday 10<sup>th</sup> October= ==Lab work== ===BioBrick K2039000 and K2039001 characterization=== ====Protein extraction of FRB - GF...") |
(→Protein extraction of FRB - GFP 11 in pSB1C3 (pPS16_019) clone 4, FKBP - GFP 10 in pSB1C3 (pPS16_018) clone 6, GFP 1.9 in pUC19 (pPS16_009) clone 1 and PhB1040 strain 594) |
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− | In order to perform a Western Blot, the proteins were extracted from clones (pPS16_018 clone 6, pPS16_019 clone 4, pPS16_009 clone 1 and PhB1040 strain 594) put on liquide culture the 7th October. For that purpose, | + | In order to perform a Western Blot, the proteins were extracted from clones (pPS16_018 clone 6, pPS16_019 clone 4, pPS16_009 clone 1 and PhB1040 strain 594) put on liquide culture the 7th October. For that purpose, the [[Team:Paris_Saclay/Experiments#Protein_extraction_from_bacterial_liquid_culture_for_Western_Blot|protein extraction protocol]] was used: |
− | + | ||
− | + | ||
+ | {| class="wikitable" | ||
+ | |- | ||
+ | !Sample | ||
+ | !OD (1 ml) | ||
+ | !Volume of lysis buffer | ||
+ | |- | ||
+ | |FRB - GFP 11 in pSB1C3 (pPS16_019) | ||
+ | |5.1 | ||
+ | |127.5 μl | ||
+ | |- | ||
+ | |FKBP - GFP 10 in pSB1C3 (pPS16_018) | ||
+ | |5.2 | ||
+ | |130 μl | ||
+ | |- | ||
+ | |GFP 1.9 in pUC19 (pPS16_009) | ||
+ | |5.3 | ||
+ | |132.5 μl | ||
+ | |- | ||
+ | |PhB1040 strain 594 | ||
+ | |2.8 | ||
+ | |70 μl | ||
+ | |- | ||
+ | |} | ||
Revision as of 13:14, 12 October 2016