Difference between revisions of "Team:Paris Saclay/Notebook/June/23"

(Transformed cell cultures)
(Lab work)
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{{Team:Paris_Saclay/notebook_header}}
 
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=Thursday 23<sup>rd</sup> June=
 
=Thursday 23<sup>rd</sup> June=
==Lab work==
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=Lab work=
  
 
====Heat-shock competent cells====
 
====Heat-shock competent cells====
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''By Lea''
 
''By Lea''
  
One colony from each transformation (device 2, device 3, negative control and positive control) obtained will be test in FACS. One colony was taken into 3.5mL LB medium + Cm at 30µg/mL.  
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One colony from each transformation (device 2, device 3, negative control and positive control) obtained will be test in FACS. One colony for each was taken into 3.5mL LB medium + Cm at 30µg/mL.  
  
 
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{{Team:Paris_Saclay/notebook_footer}}

Revision as of 12:56, 9 October 2016

Thursday 23rd June

Lab work

Heat-shock competent cells

By Caroline and Lea

The cells competency was checked by transforming cells as usual with two control plasmids:

  • the Interlab study negative control
  • pSB1C3 blue

Interlab study

Transformation results

By Caroline, Lea and Marion

Colonies were observed for all transformations except for device 1 and positive control. The transformation was redone for device 1 with 5µL and the rest of the transformed cells obtained on 22/06/2016 were spread entirely.

Transformed cell cultures

By Lea

One colony from each transformation (device 2, device 3, negative control and positive control) obtained will be test in FACS. One colony for each was taken into 3.5mL LB medium + Cm at 30µg/mL.