Difference between revisions of "Team:Paris Saclay/Notebook/July/6"

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===Biobrick characterization===
 
===Biobrick characterization===
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==== DNA extraction of K1372001 clone 1 and clone 2====
''By ''
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''By Mathilde''
  
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Plasmids DNA were extracted following the [[Team:Paris_Saclay/Experiments#PlasmidExtraction|usual]] protocol except that isopropanol was used instead of 100% ethanol and that pellets were dried on the lab bench top instead of in the speedvac. The extraction were kept at -20°C.
  
 
===Visualization===
 
===Visualization===

Revision as of 15:01, 18 July 2016

Wednesday 6th July

Lab work

Preparation of LB solid and liquid stock

By Léa, Naiane, Laetitia

- 2L of LB liquid : 20g/L powder LB + 2L water μQ
- 1L of LB solid : 1L of LB liquid +15g/L of Agar
The all was put in the autoclave for sterilization with the help of Sylvain.

Biobrick characterization

DNA extraction of K1372001 clone 1 and clone 2

By Mathilde

Plasmids DNA were extracted following the usual protocol except that isopropanol was used instead of 100% ethanol and that pellets were dried on the lab bench top instead of in the speedvac. The extraction were kept at -20°C.

Visualization

gBlocks PCR screening

By Caroline

A PCR screening was carry out on the bactaria transformed with the gBlocks (6 clones for each except pPS16_004 for which it was only 2 clones). We use the Taq usual protocol. We used puc19 universal primers 1151_pheoR and 1152_pheoF.

1 μL of each culture were added for each Gblock pPS16_001, pPS16_002, pPS16_003, pPS16_004, pPS16_005, pPS16_006 and pPS16_007.

pPS16_004, pPS16_007 and pPS16_007 clone 6 (already selected the 1/07/2016) streaks

By Léa, Caroline and Laetitia

The transformations carried out the 30/06/2016 were streaked again on LB petri dishes containing 50µg/ml of ampliciline on which XGal/IPTG diluted at 1/1000 was spread on (white/blue sreen). The petri dishes were incubated ON at 37°C.