Difference between revisions of "Team:Paris Saclay/Notebook/July/18"

(Monday 18th July)
(Lab work)
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=Monday 18<sup>th</sup> July=
 
=Monday 18<sup>th</sup> July=
 
==Lab work==
 
==Lab work==
 +
====Preparation of LB solid and liquid stock====
 +
''By Caroline and Térrence''
 +
 +
- 1L of LB liquid : 20g/L powder LB + 1L water μQ<br/>
 +
- 500mL of LB solid : 500mL of LB liquid + 7.5g/L of Agar<br/>
 +
The all was put in the autoclave for sterilization.
  
 
===Getting DNA closer===
 
===Getting DNA closer===

Revision as of 15:05, 18 July 2016

Monday 18th July

Lab work

Preparation of LB solid and liquid stock

By Caroline and Térrence

- 1L of LB liquid : 20g/L powder LB + 1L water μQ
- 500mL of LB solid : 500mL of LB liquid + 7.5g/L of Agar
The all was put in the autoclave for sterilization.

Getting DNA closer

Electrophoresis of PCR products DS_SPcasN, DS_TDcasN, pZA21 and pZA31

By Caroline

PCR products obtained the 13/07/2016 were put to migrate for 30min in a 0,8% agarose gel.

Biobrick characterization

By

Visualization

Electrophoresis of PCR products pPS16_004

By Caroline

PCR products obtained the 13/07/2016 were put to migrate for 30min in a 0,8% agarose gel