Difference between revisions of "Team:Paris Saclay/Notebook/July/20"

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(pclTAA, pclTAG and pclTq transformations)
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==== pclTAA, pclTAG and pclTq transformations====
 
==== pclTAA, pclTAG and pclTq transformations====
 
''By Leatitia and Caroline''
 
''By Leatitia and Caroline''
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HeatShock competent cells were transformed following the [[Team:Paris_Saclay/Experiments#heat-shocktransformation|usual protocol]] with pclTAA, pclTAG and pclTq. A control was made with cells without plasmid.
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Cells were plated on LB + Streptomycin (50µg/ml) and incubated overnight at 37°C.
  
 
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Revision as of 10:01, 20 July 2016

Wednesday 20th July

Lab work

By


Biobrick characterization

pclTAA, pclTAG and pclTq transformations

By Leatitia and Caroline

HeatShock competent cells were transformed following the usual protocol with pclTAA, pclTAG and pclTq. A control was made with cells without plasmid. Cells were plated on LB + Streptomycin (50µg/ml) and incubated overnight at 37°C.

By


Visualization

By


By


By


Get DNA Closer

Electrophoresis of PCR products DS_SPcasN, DS_TDcasN and pZA21

By Caroline

PCR products obtained the 19/07/2016 were put to migrate for 30min in a 0,8% agarose gel.

pZA21 transformation

By Leatitia and Caroline