Wednesday 20^th July

Lab work

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Biobrick characterization

pclTAA, pclTAG and pclTq transformations

By Leatitia and Caroline

HeatShock competent cells were transformed following the usual protocol with pclTAA, pclTAG and pclTq. A control was made with cells without plasmid. Cells were plated on LB + Streptomycin (50µg/ml) and incubated overnight at 37°C.

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Visualization

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Get DNA Closer

Electrophoresis of PCR products DS_SPcasN, DS_TDcasN and pZA21

By Caroline

PCR products obtained the 19/07/2016 were put to migrate for 30min in a 0,8% agarose gel.

pZA21 transformation

By Leatitia and Caroline

In order to restore our plasmids stocks heatShock competent cells were transformed following the usual protocol with pZA21. A control was made with cells without plasmid. The transformed bacteria resulting will be kept on glycerol. Cells were plated on LB + Kanamycin (50µg/ml) and incubated overnight at 37°C.