Difference between revisions of "Team:Paris Saclay/Notebook/August/2"

(DreamTap PCR on DH5alp|pPS16_001, DH5alp|pPS16_002, DH5alp|pPS16_005, DH5alp|pPS16_009, DH5alp|pPS16_0010, DH5alp|pPS16_011, DH5alp|pPS16_012, DH5alp|pPS16_013, DH5alp|pPS16_014, DH5alp|pPS16_015)
(Monday 1st August)
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{{Team:Paris_Saclay/notebook_header}}
 
{{Team:Paris_Saclay/notebook_header}}
= Monday 1<sup>st</sup> August=
+
= Tuesday 2<sup>st</sup> August=
 
==Lab work==
 
==Lab work==
 
===Visualization===
 
===Visualization===

Revision as of 09:34, 2 August 2016

Tuesday 2st August

Lab work

Visualization

DreamTap PCR on DH5alp|pPS16_001, DH5alp|pPS16_002, DH5alp|pPS16_005, DH5alp|pPS16_009, DH5alp|pPS16_0010, DH5alp|pPS16_011, DH5alp|pPS16_012, DH5alp|pPS16_013, DH5alp|pPS16_014, DH5alp|pPS16_015

By Charlène, Mathilde, Laetitia, Caroline and Léa

A Low fidelity PCR was carried out following the usual protocol and using the universal puc19 primers. 6 clones for each transformations were tested.

Sequencing of the gBlock 4.2

By Caroline

The PCR products obtain with Q5 on pPS16_008 were send to sequencing.