Tuesday 3^st August

Lab work

Visualization

Glycerol stocks

By Caroline

The bacteria transformed with the plasmids sent the 2/08/2016 and those that would be sent the 4/08/2016 were put into glycerol. 1mL of liquid culture and 0.5mL of glycerol were put at -20°C.

High fidelity Q5 PCR on DH5alp|pPS16_004, DH5alp|pPS16_006 and DH5alp|pPS16_007

By Caroline

Q5 PCR was carried out following the usual protocol adapted to 50µL at a TM of 72°C. The primers used were specific to amplify only the interested sequence.

By

Extraction of the plasmids containing gBlocks

By Naiane

The usual protocol was used to extract plasmidic DNA of pPS16_003 (Gblock 2.1), pPS16_004 (Gblock 2.2), pPS16_006 (Gblock 3.2) and pPS16_007 (Gblock 4.1) from 3mL of overnight culture. Plasmids were resuspended in 100μL of Milli-Q water.

Interlab Study

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