Tuesday 3^st August

Lab work

Visualization

Glycerol stocks

By Caroline

The bacteria transformed with the plasmids sent the 2/08/2016 and those that would be sent the 4/08/2016 were put into glycerol. 1mL of liquid culture and 0.5mL of glycerol were put at -20°C.

High fidelity Q5 PCR on DH5alp|pPS16_004, DH5alp|pPS16_006 and DH5alp|pPS16_007

By Caroline

Q5 PCR was carried out following the usual protocol adapted to 50µL at a TM of 72°C. The primers used were specific to amplify only the interested sequence.

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Extraction of the plasmids containing gBlocks

By Naiane

The kit "Charge Switch-Pro Plasmid Miniprep" was used to extract plasmidic DNA of pPS16_003 (Gblock 2.1), pPS16_004 (Gblock 2.2), pPS16_006 (Gblock 3.2) and pPS16_007 (Gblock 4.1) from 3mL of overnight culture. Plasmids were resuspended in 100μL of Milli-Q water.

Interlab Study

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