Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Thursday 14th October 1.1 Visualization 1.1.1 Plasmids extraction of FRB - GFP 11 - FKBP - GFP 10 in pSB1C3 (pPS16_021) and GFP 1.9 in pSB1C3 (pPS16_020) transformed the 11th October 1.1.2 Digestion of FRB - GFP 11 - FKBP - GFP 10 in pSB1C3 (pPS16_021) and GFP 1.9 in pSB1C3 (pPS16_020) transformed the 11th October Thursday 14th October Visualization Plasmids extraction of FRB - GFP 11 - FKBP - GFP 10 in pSB1C3 (pPS16_021) and GFP 1.9 in pSB1C3 (pPS16_020) transformed the 11th October By Sylvie The following plasmids were extracted using a "standard Plasmid Miniprep": pPS16_020 (FKBP - GFP 10) pPS16_021 (FRB - GFP 11) Digestion of FRB - GFP 11 - FKBP - GFP 10 in pSB1C3 (pPS16_021) and GFP 1.9 in pSB1C3 (pPS16_020) transformed the 11th October Extracted pPS16_020 and pPS16_021 were digested by restriction enzyme NotI: 4 µL of plasmid 2 µL of buffer Tango 1 µL of restriction enzyme NotI 13 µL of water The mix were incubated for 30 minutes at 37°C. Then, 15 µL of digestion products and 5 µL of DNA ladder were placed in wells and migrated at 100V during 30 min. GEL SYLVIE 6
By Sylvie
The following plasmids were extracted using a "standard Plasmid Miniprep":
Extracted pPS16_020 and pPS16_021 were digested by restriction enzyme NotI:
The mix were incubated for 30 minutes at 37°C. Then, 15 µL of digestion products and 5 µL of DNA ladder were placed in wells and migrated at 100V during 30 min.
GEL SYLVIE 6
