Toggle navigation Home Team Team Media Collaborations Sponsors Acknowledgements Project Background Design CRISPR/Cas9 Strategy Experiments Notebook Results Perspective Interlab Study Parts Parts Basic Parts Composite Parts Human Pratices Overview Societal Issues of CRISPR/Cas9 Responsible Research and Innovation GMO regulation Integrated Practices Engagement Model Attributions Safety Contents 1 Wednesday 6th July 1.1 Lab work 1.1.1 Preparation of LB solid and liquid stock 1.1.2 Bringing DNA closer 1.1.2.1 1.1.3 Biobrick characterization 1.1.3.1 1.1.4 Visualization 1.1.4.1 gBlocks PCR screening 1.1.4.2 pPS16_004, pPS16_007 and pPS16_007 clone 6 (already selected the 1/07/2016) streaks Wednesday 6th July Lab work Preparation of LB solid and liquid stock By Léa, Naiane, Laetitia - 2L of LB liquid : 20g/L powder LB + 2L water μQ - 1L of LB solid : 1L of LB liquid +15g/L of Agar The all was put in the autoclave for sterilization with the help of Sylvain. Bringing DNA closer By Biobrick characterization By Visualization gBlocks PCR screening By Caroline A PCR screening was carry out on the bactaria transformed with the gBlocks (6 clones for each except pPS16_004 for which it was only 2 clones). We use the Taq usual protocol. We used puc19 universal primers 1151_pheoR and 1152_pheoF. 1 μL of each culture were added for each Gblock pPS16_001, pPS16_002, pPS16_003, pPS16_004, pPS16_005, pPS16_006 and pPS16_007. pPS16_004, pPS16_007 and pPS16_007 clone 6 (already selected the 1/07/2016) streaks By Léa, Caroline and Laetitia The transformations carried out the 30/06/2016 were streaked again on LB petri dishes containing 50µg/ml of ampliciline on which XGal/IPTG diluted at 1/1000 was spread on (white/blue sreen). The petri dishes were incubated ON at 37°C.
By Léa, Naiane, Laetitia
- 2L of LB liquid : 20g/L powder LB + 2L water μQ - 1L of LB solid : 1L of LB liquid +15g/L of Agar The all was put in the autoclave for sterilization with the help of Sylvain.
By
By Caroline
A PCR screening was carry out on the bactaria transformed with the gBlocks (6 clones for each except pPS16_004 for which it was only 2 clones). We use the Taq usual protocol. We used puc19 universal primers 1151_pheoR and 1152_pheoF.
1 μL of each culture were added for each Gblock pPS16_001, pPS16_002, pPS16_003, pPS16_004, pPS16_005, pPS16_006 and pPS16_007.
By Léa, Caroline and Laetitia
The transformations carried out the 30/06/2016 were streaked again on LB petri dishes containing 50µg/ml of ampliciline on which XGal/IPTG diluted at 1/1000 was spread on (white/blue sreen). The petri dishes were incubated ON at 37°C.
